Cytometry Core Facility

National Jewish Health
K1014 Goodman Building
Phone: (303) 398-1330 FAX (303) 398-1225
Email: beltrig @njc.org Webpage http://flowlab.njc.org/

Instrument	Main Uses
CYAN 405nm, 488 , 630nm lasers	rapid 9 color fluorescence
FACSscan with Rainbow 488, 630	up to 5 color analysis
FACScalibur with 640 nm excitation	up to 4 color analysis
BD LSR Argon, HeNe, HeCd	up to six colors [Ca++] flux
MoFlo with visable,uv, and 633nm	rapid cell sorting, up to eight color
MoFloII with visable, and 633nm	rapid cell sorting, up to 4 color
Imagestream 100	flow imaging
Signal Analytics IP Lab imaging	longworking distance fluorescence
Leica-Slidebook scope	4 color slides and DIC images, z
Marianas Slidebook scope	multicolor,z,live cell,Ca++ flux
Neustadt slidebook	inverted multicolor, z sectioning
T.I.L.L. Olympus	rapid multicolor scope for live cell imaging
Long working distance Marianas	long working distance dry objectives
Olympus/Apogee	brightfield absorption stained slides
Zeiss confocal/multiphoton	deep tissue imaging, FRAP

Analysis Available on Computer Systems
TILLvisION	TILL data
ImageJ	NIH image analysis program IBM
Cell Quest	FCS data on a MAC
Mcycle	DNA data on a IBM
Modfit	DNA data on a MAC
Mtime	time course experiments like [Ca++]
Repromac	FCS data on MAC for extra graphics applications
NIH Image	microscope data on MAC
Real-14	microscope data on IBM
Iplab	microscope data on MAC
Slidebook	microscope data on MAC + IBM
FloJo	flow data on MAC
IDEAS	Imagestream analysis
LSM 510	Zeiss confocal analysis software

Staff
John Cambier	Scientific Administrator
Bill Townend	Facility Manager
Shirley Sobus	Operator and billing
Joshua Loomis	Operator
Jen Bickert	Assistant